signal peptide sequence造句
例句與造句
- Expression of crylac in transgenic tobacco plants was assayed with elisa . the results showed that pinll signal peptide sequence enhanced the expression of crylac protein in transgenic tobacco
熒光顯微觀察到gfp在植物細(xì)胞間隙高效表達(dá)， westernblot結(jié)果顯示crylac蛋白也在植物細(xì)胞間隙表達(dá)。 - Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco . the results of fluorescent detection showed gfp localization in the apoplast
Elisa結(jié)果顯示，馬鈴薯蛋白酶抑制劑基因的信號(hào)肽序列使crylac基因在轉(zhuǎn)基因煙草中的表達(dá)量顯著提高。 - We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0
我們直接將含有4個(gè)內(nèi)含子和自身信號(hào)肽的2 . 4kb全長(zhǎng)人生長(zhǎng)激素基因直接克隆至真核表達(dá)載體pcdna3 . 0 ，然后利用脂質(zhì)體轉(zhuǎn)染家蠶bmn細(xì)胞，瞬時(shí)表達(dá)hgh 。 - To allow secretion of the crylac protein into the intercellular space , potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac
運(yùn)用pcr技術(shù)克隆了馬鈴薯蛋白酶抑制劑基因的信號(hào)肽序列，并將其分別連到crylac 、 gfp基因的5 ’端，構(gòu)建植物轉(zhuǎn)化載體p3301ubisigac和p3301ubisiggfp 。 - In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ) , nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence
在用煙草作為生物反應(yīng)器時(shí)，分別將該單鏈抗體靶向細(xì)胞質(zhì)和內(nèi)質(zhì)網(wǎng)。經(jīng)westernblot分析，靶向細(xì)胞質(zhì)中表達(dá)時(shí)，可溶單鏈抗體最高占總的可溶蛋白的0 . 06 。 - It's difficult to find signal peptide sequence in a sentence. 用signal peptide sequence造句挺難的
- ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
( 3 )翻譯后水平通過(guò)pcr擴(kuò)增的方式在t - pa基因5端添加了真核分泌信號(hào)肽序列和植物翻譯起始共有序列aaca ，在3端添加了內(nèi)質(zhì)網(wǎng)定位序列kdel ，構(gòu)建了植物表達(dá)載體pbemt 。 - The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence , which interdicted the process of protein entering golgi body and cytoplasm , and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism . 2
真核分泌信號(hào)肽序列可以引導(dǎo)新合成的蛋白質(zhì)進(jìn)入內(nèi)質(zhì)網(wǎng)腔， kdel序列將進(jìn)入內(nèi)質(zhì)網(wǎng)腔的蛋白質(zhì)錨定在內(nèi)質(zhì)網(wǎng)內(nèi)壁上，從而阻斷了蛋白質(zhì)進(jìn)入高爾基體和細(xì)胞質(zhì)的過(guò)程，進(jìn)而避免了外源蛋白質(zhì)的異源糖基化修飾，延長(zhǎng)了蛋白質(zhì)在生物體內(nèi)的半衰期。 - The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene , this intron contains donor sequence - gtatgc , lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene . this gene encodes a peptide of 467amino acid residues with molecular weight of 51 . 37kda , containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明， pcr擴(kuò)增產(chǎn)物中包含有完整的phya基因，該基因全長(zhǎng)1506bp ，其中包含一段長(zhǎng)102bp的內(nèi)含子，該內(nèi)含子具有真菌植酸酶基因內(nèi)含子的特征保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個(gè)氨基酸，理論分子量為51 . 37kda ，其上有13個(gè)潛在的n -糖基化位點(diǎn)， n端19個(gè)氨基酸為信號(hào)肽序列，植酸酶活性位點(diǎn)序列( crvtfaqvlsrhgaryptdskgk )位于氨基酸序列的+ 71 + 93 。 - In comparison with genbank data , the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98 . 4 % and 100 % ; ha was 97 . 2 % and 99 . 3 % respectively . 4 . two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon , leader signal peptide sequence and termination codon
序列分析表明，所克隆獲得的基因與genbank中已經(jīng)登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗(yàn)蟲(chóng)株與國(guó)外報(bào)道的同源性很高。 - Therefore , blys , its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity , neoplasia , or immunodeficiency syndromes . in this study , epo signal peptide sequence and hsblys gene were linked by soe method . the fusion product was cloned into eukaryotic plasmids . pcdna3 , pcdna3 . 1 , pefneo , respectively . meanwhile , the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i . thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本實(shí)驗(yàn)通過(guò)3 ’端互補(bǔ)，進(jìn)行引物延伸合成epo信號(hào)肽序列：信號(hào)肽和hsblys基因采用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號(hào)肽時(shí)，利用亮氨酸同義密碼，將信號(hào)肽基因的倒數(shù)第二個(gè)密碼突變，在重組載體上的信號(hào)肽序列之后，形成bln酶切位點(diǎn)，使三種載體成為分泌表達(dá)載體。 - The signal peptide sequence which was used for secretedly expressing divergent gene in mammary gland cells was ligated to the e2 gene , the e2 gene with signal sequence was obtained by pcr . the gene resisting kanamycin was cut down from pgfp - cl vector and inserted into p22 vector , a p22 vector with gene resisting kanamycin was constructed , it was tried to construct an expression vector for transforming go
豬瘟病毒ez基因的乳腺特異表達(dá)載體構(gòu)建：將在乳腺細(xì)胞中特異分泌的信號(hào)肽序列連接到ez基因， pcr得到了目的片段，再將pgfpci載體上的kana基因切下與在乳腺細(xì)胞中特異表達(dá)的p22載體連接，使其帶有篩選標(biāo)記，然后將帶有信號(hào)肽的ez插入到p22中，試圖構(gòu)建山羊乳腺上皮細(xì)胞的特異性表達(dá)載體。